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Item Details
Title: FIBROSIS RESEARCH
METHODS AND PROTOCOLS
By: John Varga (Editor), David A. Brenner (Editor), Sem H. Phan (Editor)
Format: Paperback

List price: £139.99
Our price: £122.49
Discount:
12.5% off
You save: £17.50
ISBN 10: 1617375926
ISBN 13: 9781617375927
Availability: Usually dispatched within 1-3 weeks.
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Publisher: HUMANA PRESS INC.
Pub. date: 28 October, 2010
Edition: 1st ed. Softcover of orig. ed. 2005
Series: Methods in Molecular Medicine 117
Pages: 392
Synopsis: Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Illustrations: 80 black & white illustrations, biography
Publication: US
Imprint: Humana Press Inc.
Returns: Returnable
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