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Item Details
Title: BACULOVIRUS EXPRESSION VECTORS
A LABORATORY MANUAL
By: David R. O'Reilly, Lois K. Miller, Verne A. Luckow
Format: Spiral bound

List price: £222.50


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ISBN 10: 0195091310
ISBN 13: 9780195091311
Publisher: OXFORD UNIVERSITY PRESS INC
Pub. date: 23 September, 1993
Pages: 360
Description: This laboratory manual simplifies selection of the most appropriate baculovirus vector design for a given problem. It then describes each step of the implementation process - from vector construction to large-scale protein production. The book provides: an understanding of how vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelines for choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods ofanalysing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors.
Synopsis: This laboratory manual simplifies selection of the most appropriate baculovirus vector design for a given problem. It then describes each step of the implementation process - from vector construction to large-scale protein production. The book provides an understanding of how the vectors work, a biological overview of cells, viruses, plasmids, and promoters, guidelines for choosing optimum vectors, protocols for growing insect cells and recombinant viruses, methods for analysing protein products and scaling up protein production, techniques for producing proteins in insect larvae, and easy to use maps charting available expression vectors.
Illustrations: halftones, line figures, tables
Publication: US
Imprint: Oxford University Press Inc
Returns: Returnable
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