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Item Details
Title: ELECTRON MICROSCOPY IN BIOLOGY
A PRACTICAL APPROACH
By: Robin Harris (Editor)
Format: Paperback

List price: £98.00


We currently do not stock this item, please contact the publisher directly for further information.

ISBN 10: 0199632154
ISBN 13: 9780199632152
Publisher: OXFORD UNIVERSITY PRESS
Pub. date: 28 November, 1991
Series: Practical Approach Series 82
Pages: 328
Description: Transmission electron microscopy is one of the most important and popular forms of microscopy used in biomedical research. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation,tissue fixation, embedding, and sectioning are covered in depth and are followed by the more specialist techniques of freeze-substitution, immunogold labelling, autoradiography, and enzyme histochemistry.
Synopsis: Transmission electron microscopy is one of the most important and widely used forms of microscopy in biomedical research. Electron Microscopy in Molecular Biology : A Practical Approach was published in 1987, and contained techniques relating specifically to nucleic acids and proteins. The present volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding, and sectioning are covered in depth and are followed by the more specialist techniques of freeze-substitution, immunogold labelling, autoradiography, and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies, and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications.It will be of interest to researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.
Illustrations: halftones, line drawings, tables
Publication: UK
Imprint: Oxford University Press
Returns: Returnable
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