|
|
|
Item Details
Title:
|
IN SITU HYBRIDIZATION IN ELECTRON MICROSCOPY
|
By: |
Gerard Morel, Annie Cavalier, Lynda Williams |
Format: |
Hardback |
List price:
|
£215.00 |
Our price: |
£193.50 |
Discount: |
|
You save:
|
£21.50 |
|
|
|
|
ISBN 10: |
0849300444 |
ISBN 13: |
9780849300448 |
Availability: |
Usually dispatched within 1-3 weeks.
Delivery
rates
|
Stock: |
Currently 0 available |
Publisher: |
TAYLOR & FRANCIS INC |
Pub. date: |
30 January, 2001 |
Series: |
Methods in Visualization |
Pages: |
472 |
Description: |
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells. This book reviews the three main methods and describes the different stages such as: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. |
Synopsis: |
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes: hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method) hybridization prior to embedding (pre-embedding) hybridization on ultrathin sections of frozen tissue (frozen tissue method). For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol.Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level. |
Illustrations: |
16 black & white tables, 126 black & white halftones |
Publication: |
US |
Imprint: |
CRC Press Inc |
Returns: |
Returnable |
|
|
|
|
Ramadan and Eid al-Fitr
A celebratory, inclusive and educational exploration of Ramadan and Eid al-Fitr for both children that celebrate and children who want to understand and appreciate their peers who do.
|
|
|
|
|
|
|
|
|
|
|