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Item Details
Title: PCR 1
A PRACTICAL APPROACH
By: M. J. McPherson (Editor), G. R. Taylor (Editor)
Format: Paperback

List price: £98.00


We currently do not stock this item, please contact the publisher directly for further information.

ISBN 10: 0199631964
ISBN 13: 9780199631964
Publisher: OXFORD UNIVERSITY PRESS
Pub. date: 5 September, 1991
Series: Practical Approach Series 77
Pages: 274
Description: Another extremely important title. The value of Polymerase Chain Reaction (PCR) cannot be over-stated. It has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnosticmedicine, genetic engineering, and the food industry. PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. The emphasis throughout is on the practical applications of the technique and detailedexperimental protocols are included at all stages. A starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.
Synopsis: The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it have been developed for different applications, for use in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry. PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, backed up by advice on avoiding potential pitfalls.This volume provides a starting point for those new to PCR and will also serve as a useful reference for more experienced researchers in all areas of biological science.
Illustrations: numerous halftones, line drawings, tables
Publication: UK
Imprint: Oxford University Press
Returns: Returnable
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